L’induction des mécanismes de défense chez la tomate

Table des matières

Chapter I: Introduction, working hypothèses and research objectives
1.1 Introduction
1.2 BTH as an efficient inducer of plant défense responses
1.2.1 The mode of action of BTH
1.2.2 The efficacy of BTH
1.3 Défense signalling pathways in plants
1.3.1 SA-mediated résistance
1.3.1.1 SA and the induction of ISR
1.3.1.2 SA biosynthesis
1.3.1.3 SA signalling in plants
1.3.2 JA-mediated résistance
1.3.2.1 The rôle of jasmonates in tomato défense
1.3.2.2 Jasmonate biosynthesis
1.3.2.3 Jasmonate signalling
1.3.3 Interacting défense pathways in planta
1.3.4 Intégration and coordination of défense responses
1.4 The “omics” expérimental approaches and the understanding of complex défense pathways in plants
1.4.1 The genomics approach
1.4.2 The proteomics approach
1.4.3 Proteomics as a complément to genomics
1.5 Research problematic
1.6 Goals, working hypothèses and research objectives
1.6.1 Working hypothesis
1.6.2 Objectives and methodological approach
1.6.2.1 Spécifie objectives
1.6.2.2 Some information on the SELDI TOF MS technology.
1.6.2.3 Some information on 2-DE based, classical proteomics
Chapter II A SELDI TOF MS procédure for the détection and monitoring of conflicting and synergistic défense responses in plants
2.1 Introductory remarks
2.2 Résumé
2.3 Abstract
2.4 Introduction
2.5 Materials and methods
2.5.1 Plant material and elicitor treatments
2.5.2 Leaf protein extraction and sample processing
2.5.3 SELDI TOF MS and data analysis
2.6 Results and discussion
2.6.1 Time-course effects of BTH on the leaf low-molecular-weight proteome
2.6.2 Differential impact of MeJA on spécifie BTH effects
Chapter III Modulation of the leaf proteome in tomato (Solanum lycopersicum) challenged with the synthetic elicitor benzothiadiazole
3.1 Introductory remarks
3.2 Résumé
3.3 Abstract
3.4 Introduction
3.5 Materials and methods
3.5.1 Plant material and elicitor treatments
3.5.2 Gene probes and northern blot analysis
3.5.3 Sample préparation and 2-DE
3.5.4 Image acquisition and data analysis
3.5.5 Protein identification
3.6 Results and discussion
3.6.1 Induction of Pin-ll and protein P4 synthesis in tomato Ieaves
3.6.2 BTH induces both SA-inducible and JA-inducible proteins in
tomato leaves
3.6.3 MeJA potentiates the inducing effects of BTH for several BTH-sensitive proteins
3.7 Conclusion
Chapter IV Conclusion and further perspectives
4.1 Conclusion
4.1.1 Objective 1
4.1.2 Objective 2
4.2 Confirmation of the working hypothèses
4.3 Future perspectives
Références