Les enzymes lipolytiques

Table des matières

CHAPITRE 1 INTRODUCTION
1.1 Les enzymes
1.2 Structure et stabilité des protéines
1.3 Les enzymes lipolytiques
1.4 Les lipases
1.5 Principes d’enzymologie
1.6 Détection de l’ activité lipase
1.7 Applications industrielles des lipases
1.8 Synthèse de biodiésel
1.9 Projet de recherche
CHAPITRE II
DEVELOPMENT OF A HIGH THROUGHPUT LIQUID STATE ASSAY FOR LIPASE ACTIVITY USING NATURAL SUBSTRATES AND RHODAMINE 
2.1 Contribution des auteurs
2.2 Résumé de l’article
2.3 Premier article scientifique
Abstract
Introduction
Materials and methods
Chemical and reagents
Liquid reaction mixture preparation
Activity assay and fatty acids standard curve
Colorimetric assay using p-nitrophenyl ester
Results and discussion
Determination of lipase activity using fluorescence from RhB olive
oil emulsions
RhB-OOe lipase activity detection under various conditions
Determination oflipase activity using RhB-OOe method
Conclusion
Acknowledgments
References
CHAPITRE III
CHARACTERIZATION OF A NOVEL ALKALOPHILIC LIPASE FROM ANEURINIBACILLUS THERMOAEROPHILUS: LID HETEROGENEITY AND ASSIGNMENT TO FAMILY 1.5
3.1 Contribution des auteurs
3.2 Résumé de l’article
3.3 Deuxième article scientifique
Abstract
Introduction
Materials and methods
Cloning of PCR products
Expression, purification and tag cleavage of recombinant LipA T
In silico analysis
Circular dichroism spectroscopy
Fluorescence spectroscopy
Dynamic light scattering analysis
Enzyme activity measurement
Lipase thermal stability measurements
Effect of metal ions
Biodiesel synthesis assay
Results and discussion
Production and purification of LipA T enzyme
Biophysical characterisation of LipA T
Enzymatic characterization
Potential industrial application: Biodiesel synthesis
In silico characterization
Conclusion
Acknowledgments
Declaration of interest
Funding information
Author contribution statement
References
CHAPITRE IV
DISCUSSION