Le follicule ovarien

Table des matières

RESUME
ABSTRACT
LISTE DES TABLES
LISTE DES FIGURES
LISTE DES ABREVIATIONS ET DES SIGLES
REMERCIEMENTS
AVANT-PROPOS
1. INTRODUCTION
1.1. Le follicule ovarien
1.1.1. Ovogenèse
1 1.1.2. Folliculogenèse
1.1.3. Ovulation
1.2. Le cumulus oophorus
1.2.1. Différenciation des cellules du cumulus, rôle de l’ovocyte
1.2.2. Communications bidirectionnelles
1.2.3. Métabolisme : missions du cumulus
1.2.4. Réponse du COC au pic préovulatoire de LH
1.2.5. Cumulus post-ovulatoire
1.3. La compétence ovocytaire au développement
1.3.1. Définition
1.3.2. Évaluation de la compétence ovocytaire
1.3.3. Mesure de la compétence ovocytaire
1.3.4. Optimisation de la stimulation ovarienne
1.4. Hypothèse et objectifs
2. CUMULUS CELL GENE EXPRESSION ASSOCIATED WITH PRE-OVULATORY ACQUISITION OF DEVELOPMENTAL COMPETENCE IN BOVINE OOCYTES
2.1. Résumé
2.2. Abstract
2.3. Introduction
2.4. Results
2.4.1. COC morphology
2.4.2. Genes inventory
2.4.3. Hierarchical cluster analysis
2.4.4. Variation of gene expression across time
2.4.5. Ingenuity Pathway Analysis (IPA)
2.4.6. qRT-PCR results
2.5. Discussion
2.6. Conclusion
2.7. Materials and methods
2.7.1. Ovarian stimulation treatment, in vitro production and cumulus–oocyte complex (COC) recovery from super-stimulated animals
2.7.2. Cumulus cell retrieval
2.7.3. RNA extraction
2.7.4. RNA processing for microarray analysis
2.7.5. Microarray data normalization and statistical analysis
2.7.6. RNA processing for qRT-PCR and statistical analysis
2.7.7. Statistical analysis of COC morphology
2.8. References
2.9. Tables
2.10. Figures
3. THE EFFECTS OF BASAL LH INHIBITION WITH CETRORELIX ON CUMULUS CELL GENE EXPRESSION DURING THE LUTEAL PHASE UNDER OVARIAN COASTING STIMULATION IN CATTLE.
3.1. Résumé
3.2. Abstract
3.3. Introduction
3.4. Results
3.4.1. COC Morphology
3.4.2. Genes Inventory and Clustering
3.4.3. IPA analysis
3.4.4. qRT-PCR results
3.5. Discussion
3.6. Conclusion
3.7. Materials and methods
3.7.1. Ovarian stimulation treatment, in vitro production, and recovery of cumulus-oocyte complexes (COC) from super-ovulated animals
3.7.2. Cumulus cell retrieval
3.7.3. RNA extraction
3.7.4. RNA processing for microarray analysis
3.7.5. Microarray data normalization and statistical analysis
3.7.6. RNA processing for qRT-PCR and statistical analysis
3.7.7. Statistical Analysis of COC Morphology
3.8. References
3.9. Tables
3.10. Figures
4. ANALYSIS OF LHCGR AND SELECTED STEROID ENZYME MRNA EXPRESSION IN BOVINE CUMULUS CELLS DURING IN VITRO MATURATION.
4.1. Résumé
4.2. Abstract
4.3. Introduction
4.4. Results
4.4.1. LHCGR and MVK mRNA expression during in vitro maturation
4.4.2. Steroidogenic enzyme and progesterone receptor mRNA expression during in vitro maturation
4.5. Discussion
4.6. Conclusion
4.7. Materials and methods
4.7.1. Cumulus-Oocyte Complex in vitro maturation and retrieval
4.7.2. RNA processing and quantification
4.8. References
4.9. Tables
4.10. Figures
5. INDIVIDUAL BOVINE
I N V I T R O EMBRYO PRODUCTION AND CUMULUS CELL TRANSCRIPTOMIC ANALYSIS TO DISTINGUISH CUMULUS-OOCYTE COMPLEXES WITH HIGH OR LOW DEVELOPMENTAL POTENTIAL
5.1. Résumé
5.2. Abstract
5.3. Introduction
5.4. Results
5.4.1. In vitro embryo production and quality assessment
5.4.2. Gene inventory
5.4.3. Ingenuity Pathway Analysis
5.4.4. Quantitative reverse transcription-polymerase chain reaction results
5.5. Discussion
5.6. Conclusion
5.7. Materials and methods
5.7.1. In vitro culture and CC recovery
5.7.2. Analysis of RNA
5.8. Acknowledgments
5.9. References
5.10. Tables
5.11. Figures
6. CONCLUSION ET DISCUSSION GENERALES
BIBLIOGRAPHIE